RESUMO
BACKGROUND: Chronic prostatitis has been supposed to be associated with preneoplastic lesions and cancer development. The objective of this study was to examine how chronic inflammation results in a prostatic microenvironment and gene mutation in C57BL/6 mice. METHODS: Immune and bacterial prostatitis mouse models were created through abdominal subcutaneous injection of rat prostate extract protein immunization (EAP group) or transurethral instillation of uropathogenic E. coli 1677 (E. coli group). Prostate histology, serum cytokine level, and genome-wide exome (GWE) sequences were examined 1, 3, and 6 months after immunization or injection. RESULT: In the EAP and E. coli groups, immune cell infiltrations were observed in the first and last months of the entire experiment. After 3 months, obvious proliferative inflammatory atrophy (PIA) and prostatic intraepithelial neoplasia (PIN) were observed accompanied with fibrosis hyperplasia in stroma. The decrease in basal cells (Cytokeratin (CK) 5+/p63+) and the accumulation of luminal epithelial cells (CK8+) in the PIA or PIN area indicated that the basal cells were damaged or transformed into different luminal cells. Hic1, Zfp148, and Mfge8 gene mutations were detected in chronic prostatitis somatic cells. CONCLUSION: Chronic prostatitis induced by prostate extract protein immunization or E. coli infection caused a reactive prostatic inflammation microenvironment and resulted in tissue damage, aberrant atrophy, hyperplasia, and somatic genome mutation.
Assuntos
Animais , Masculino , Camundongos , Lesões Pré-Cancerosas/genética , Prostatite/genética , Infecções por Escherichia coli/patologia , Mutação/genética , Lesões Pré-Cancerosas/microbiologia , Lesões Pré-Cancerosas/patologia , Prostatite/microbiologia , Prostatite/patologia , Imuno-Histoquímica , Doença Crônica , Modelos Animais de Doenças , Camundongos Endogâmicos C57BLRESUMO
Este trabajo muestra la colonización bacteriana del tejido de la glándula prostática en condiciones que asemejen a la normalidad.Realizamos un estudio prospectivo con 45 pacientes sometidos a resección transuretral. Se realizó cultivo corriente de tejido prostático, resultando positivo en 25 porciento de las muestras.Los gérmenes Gram (+) fueron lo más frecuentes.La presencia de inflamación prostática se asoció a presencia bacteriana en 22 porciento.Este hecho apoya la hipótesis de la etiología autoinmune de las prostatitis.